2Q5F
Crystal structure of LMNADK1 from Listeria monocytogenes
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-11-13 |
| Detector | ADSC QUANTUM 210 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 62.959, 75.088, 118.490 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.200 - 1.900 |
| R-factor | 0.18914 |
| Rwork | 0.188 |
| R-free | 0.22181 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB2I2C |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.430 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | REFMAC (5.2.0019) |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.200 | 1.990 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.060 | 0.489 |
| Number of reflections | 19663 | |
| <I/σ(I)> | 20.2 | 2.5 |
| Completeness [%] | 87.5 | 51.6 |
| Redundancy | 5.9 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 291 | PEG400 15-20% w/v, KCitrate 50 mM, KCl 300 mM, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
