2Q5C
Crystal structure of NtrC family transcriptional regulator from Clostridium acetobutylicum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-05-05 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 1 |
| Unit cell lengths | 45.950, 45.948, 58.284 |
| Unit cell angles | 72.94, 72.98, 82.07 |
Refinement procedure
| Resolution | 43.850 - 1.490 |
| R-factor | 0.188 |
| Rwork | 0.187 |
| R-free | 0.21000 |
| Structure solution method | SAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.430 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELX (C/D/E) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.540 |
| High resolution limit [Å] | 1.490 | 1.490 |
| Rmerge | 0.098 | 0.263 |
| Number of reflections | 68459 | |
| <I/σ(I)> | 18.4 | |
| Completeness [%] | 95.9 | 87.7 |
| Redundancy | 4.2 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 295 | 100mM Sodium Hepes pH 7.5, 2% PEG 400, 2M Ammonium sulfate, VAPOR DIFFUSION, SITTING DROP, temperature 295K, pH 7.50 |
