2Q5C
Crystal structure of NtrC family transcriptional regulator from Clostridium acetobutylicum
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-05-05 |
Detector | ADSC QUANTUM 315 |
Spacegroup name | P 1 |
Unit cell lengths | 45.950, 45.948, 58.284 |
Unit cell angles | 72.94, 72.98, 82.07 |
Refinement procedure
Resolution | 43.850 - 1.490 |
R-factor | 0.188 |
Rwork | 0.187 |
R-free | 0.21000 |
Structure solution method | SAD |
RMSD bond length | 0.013 |
RMSD bond angle | 1.430 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHELX (C/D/E) |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.540 |
High resolution limit [Å] | 1.490 | 1.490 |
Rmerge | 0.098 | 0.263 |
Number of reflections | 68459 | |
<I/σ(I)> | 18.4 | |
Completeness [%] | 95.9 | 87.7 |
Redundancy | 4.2 | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.5 | 295 | 100mM Sodium Hepes pH 7.5, 2% PEG 400, 2M Ammonium sulfate, VAPOR DIFFUSION, SITTING DROP, temperature 295K, pH 7.50 |