2Q3X
The RIM1alpha C2B domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-02-17 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 62.037, 62.037, 145.180 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 26.400 - 1.730 |
| R-factor | 0.1793 |
| Rwork | 0.178 |
| R-free | 0.21520 |
| Structure solution method | SAD |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.811 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXS |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 26.400 | 1.775 |
| High resolution limit [Å] | 1.730 | 1.730 |
| Rmerge | 0.747 | |
| Number of reflections | 34669 | |
| <I/σ(I)> | 2.1 | |
| Completeness [%] | 95.3 | 100 |
| Redundancy | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 3.5 | 293 | 1.9 M ammonium sulfate, 0.1 M sodium citrate, 0.15 M NaCl, 20% ethylene glycol, pH 3.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
