2Q3W
Ensemble refinement of the protein crystal structure of the cys84ala cys85ala double mutant of the [2Fe-2S] ferredoxin subunit of toluene-4-monooxygenase from Pseudomonas mendocina KR1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 44.510, 52.400, 83.556 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 31.430 - 1.480 |
R-factor | 0.144 |
Rwork | 0.144 |
R-free | 0.18100 |
Structure solution method | Re-refinement using ensemble model |
Starting model (for MR) | 1vm9 |
RMSD bond length | 0.020 |
RMSD bond angle | 2.000 |
Phasing software | CNS (1.1) |
Refinement software | CNS (1.1) |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 |