2Q3F
X-ray crystal structure of putative human Ras-related GTP binding D in complex with GMPPNP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-04-14 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.97942, 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 47.730, 67.130, 66.360 |
| Unit cell angles | 90.00, 110.92, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.100 |
| R-factor | 0.223 |
| Rwork | 0.221 |
| R-free | 0.26800 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.280 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.120 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Rmerge | 0.542 | |
| Number of reflections | 24803 | |
| <I/σ(I)> | 2.4 | |
| Completeness [%] | 99.8 | 99.1 |
| Redundancy | 4.1 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8.4 | 298 | 31% PEG4000, 0.2M sodium acetate and 0.1M Tris HCl, pH 8.4, VAPOR DIFFUSION, temperature 298K |
