2Q31
Actin Dimer Cross-linked Between Residues 41 and 374 and proteolytically cleaved by subtilisin between residues 47 and 48.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.2 |
Synchrotron site | ALS |
Beamline | 8.2.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-10-21 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.00 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 108.253, 71.984, 54.703 |
Unit cell angles | 90.00, 104.68, 90.00 |
Refinement procedure
Resolution | 43.070 - 2.700 |
R-factor | 0.251 |
Rwork | 0.249 |
R-free | 0.28800 |
Structure solution method | difference Fourier |
Starting model (for MR) | 2q1n |
RMSD bond length | 0.006 |
RMSD bond angle | 1.035 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 90.000 | 90.000 | 2.800 |
High resolution limit [Å] | 2.700 | 5.820 | 2.700 |
Rmerge | 0.176 | 0.091 | 0.418 |
Number of reflections | 19970 | ||
<I/σ(I)> | 5.7 | ||
Completeness [%] | 87.5 | 99.4 | 55.8 |
Redundancy | 2.8 | 3.3 | 2.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 298 | 30% MPD, 0.1 M sodium acetate, 0.01M calcium chloride, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |