2Q30
Crystal structure of a rmlc-like cupin protein (dde_2303) from desulfovibrio desulfuricans subsp. at 1.94 A resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.3 |
Synchrotron site | ALS |
Beamline | 5.0.3 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-09-10 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.00000 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 43.140, 133.950, 74.660 |
Unit cell angles | 90.00, 93.04, 90.00 |
Refinement procedure
Resolution | 29.025 - 1.940 |
R-factor | 0.169 |
Rwork | 0.167 |
R-free | 0.21200 |
Structure solution method | SAD |
RMSD bond length | 0.014 |
RMSD bond angle | 1.791 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | SHELX |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 29.025 | 29.025 | 2.010 |
High resolution limit [Å] | 1.940 | 4.170 | 1.940 |
Rmerge | 0.060 | 0.032 | 0.317 |
Number of reflections | 60310 | 10983 | 7201 |
<I/σ(I)> | 8.45 | 16.7 | 2.5 |
Completeness [%] | 81.5 | 87.1 | 57 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | NANODROP, 35.0% PEG 3000, 0.2M Sodium chloride, 0.1M Tris-HCl pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
2 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | NANODROP, 40.0% PEG 3000, 0.2M Sodium chloride, 0.1M Tris-HCl pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |