2Q2X
Crystal Structure of the ECH2 decarboxylase domain of CurF from Lyngbya majuscula
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-08-16 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.0332,0.97942,0.97956,0.95373 |
Spacegroup name | P 3 2 1 |
Unit cell lengths | 105.433, 105.433, 46.314 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 41.310 - 2.000 |
R-factor | 0.204 |
Rwork | 0.202 |
R-free | 0.25000 |
Structure solution method | MAD |
RMSD bond length | 0.011 |
RMSD bond angle | 1.245 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE (2.11) |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.063 | 0.682 |
Number of reflections | 20390 | |
<I/σ(I)> | 13.4 | 3.6 |
Completeness [%] | 99.9 | 100 |
Redundancy | 11 | 10.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.15 | 277.15 | 1.5M sodium malonate pH 7.0, 50 mM HEPES pH 6.8, 20 mM Tris pH 7.9, 500 mM NaCl, 10% glycerol, pH 7.15, VAPOR DIFFUSION, HANGING DROP, temperature 277.15K |
1 | VAPOR DIFFUSION, HANGING DROP | 7.15 | 277.15 | 1.5M sodium malonate pH 7.0, 50 mM HEPES pH 6.8, 20 mM Tris pH 7.9, 500 mM NaCl, 10% glycerol, pH 7.15, VAPOR DIFFUSION, HANGING DROP, temperature 277.15K |
1 | VAPOR DIFFUSION, HANGING DROP | 7.15 | 277.15 | 1.5M sodium malonate pH 7.0, 50 mM HEPES pH 6.8, 20 mM Tris pH 7.9, 500 mM NaCl, 10% glycerol, pH 7.15, VAPOR DIFFUSION, HANGING DROP, temperature 277.15K |