2Q2K
Structure of nucleic-acid binding protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-04-04 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.03 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 56.300, 56.300, 232.500 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 41.270 - 3.000 |
| R-factor | 0.258 |
| Rwork | 0.258 |
| R-free | 0.29700 |
| Structure solution method | MIR |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.600 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 77.600 | 3.190 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.058 | 0.372 |
| Number of reflections | 4506 | |
| <I/σ(I)> | 15 | 2.1 |
| Completeness [%] | 97.0 | 97 |
| Redundancy | 3 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | PEG 4000, isopropanol, HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG 4000 | ||
| 2 | 1 | 1 | isopropanol | ||
| 3 | 1 | 1 | HEPES | ||
| 4 | 1 | 2 | PEG 4000 | ||
| 5 | 1 | 2 | isopropanol | ||
| 6 | 1 | 2 | HEPES |
