2Q2I
Crystal structure of the protein secretion chaperone CsaA from Agrobacterium tumefaciens.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2004-08-24 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.54 |
Spacegroup name | P 61 |
Unit cell lengths | 60.653, 60.653, 113.389 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 52.530 - 1.550 |
R-factor | 0.18 |
Rwork | 0.178 |
R-free | 0.20800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1gd7 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.282 |
Data reduction software | CrystalClear |
Data scaling software | d*TREK (8.0SSI) |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 52.530 | 1.610 |
High resolution limit [Å] | 1.550 | 1.550 |
Rmerge | 0.040 | 0.341 |
Total number of observations | 35222 | |
Number of reflections | 34123 | |
<I/σ(I)> | 23.7 | 7.2 |
Completeness [%] | 99.8 | 99.6 |
Redundancy | 10.82 | 10.13 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 294 | 1.8M ammonium sulfate, 0.1M HEPES pH 7.5, 2% PEG400, 5% ethylene glycol, VAPOR DIFFUSION, SITTING DROP, temperature 294K |