2Q2C
Crystal structures of the arginine-, lysine-, histidine-binding protein ArtJ from the thermophilic bacterium Geobacillus stearothermophilus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-03-12 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.95097 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 67.070, 102.200, 70.535 |
| Unit cell angles | 90.00, 95.36, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.350 |
| R-factor | 0.27 |
| Rwork | 0.268 |
| R-free | 0.33300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.878 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.430 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rmerge | 0.139 | 0.364 |
| Number of reflections | 36922 | |
| <I/σ(I)> | 6.4 | 2.2 |
| Completeness [%] | 91.6 | 80.1 |
| Redundancy | 2.9 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | 0.2 M ammoniumsulfate, 30.5 % w/v polyethylene glycol (PEG) 2000 , pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
