2Q2A
Crystal structures of the arginine-, lysine-, histidine-binding protein ArtJ from the thermophilic bacterium Geobacillus stearothermophilus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.2 |
| Synchrotron site | BESSY |
| Beamline | 14.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-11-05 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.95373 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 71.512, 67.794, 102.676 |
| Unit cell angles | 90.00, 95.16, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.790 |
| R-factor | 0.194 |
| Rwork | 0.192 |
| R-free | 0.23700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.338 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.680 |
| High resolution limit [Å] | 1.620 | 1.620 |
| Rmerge | 0.051 | 0.191 |
| Number of reflections | 112699 | |
| <I/σ(I)> | 12 | 4.13 |
| Completeness [%] | 90.7 | 69.4 |
| Redundancy | 2.3 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | 0.2 M ammoniumsulfate, 30.5 % w/v polyethylene glycol (PEG) 2000, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
