2Q22
Crystal structure of uncharacterized protein (YP_323524.1) from Anabaena variabilis ATCC 29413 at 2.11 A resolution
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-2 |
Synchrotron site | SSRL |
Beamline | BL9-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-04-01 |
Detector | MARMOSAIC 325 mm CCD |
Wavelength(s) | 0.91837, 0.97939 |
Spacegroup name | P 32 |
Unit cell lengths | 103.374, 103.374, 42.086 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 29.841 - 2.110 |
R-factor | 0.193 |
Rwork | 0.191 |
R-free | 0.22200 |
Structure solution method | MAD |
RMSD bond length | 0.017 |
RMSD bond angle | 1.521 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | SOLVE |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 29.841 | 29.840 | 2.160 |
High resolution limit [Å] | 2.110 | 9.440 | 2.110 |
Rmerge | 0.118 | 0.051 | 0.651 |
Total number of observations | 1158 | 7880 | |
Number of reflections | 28912 | ||
<I/σ(I)> | 5.2 | 11.4 | 1.2 |
Completeness [%] | 100.0 | 96.4 | 100 |
Redundancy | 3.8 | 3.7 | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.18 | 293 | NANODROP, 5.0% PEG 1000, 48.3% Ethylene glycol, 0.1M Sodium acetate pH 4.18, VAPOR DIFFUSION, SITTING DROP, temperature 293K |