2Q20

Structure of the germline Vk1 O18/O8 light chain variable domain homodimer

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-BM
Synchrotron site	APS
Beamline	19-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2007-02-07
Detector	SBC-3
Wavelength(s)	1.0718
Spacegroup name	P 61
Unit cell lengths	74.265, 74.265, 99.051
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	99.000 - 1.300
R-factor	0.11955
Rwork	0.119
R-free	0.14815
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	polyserine model of pdb entry 1BRE chain A
RMSD bond length	0.019
RMSD bond angle	1.773
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	99.000	1.330
High resolution limit [Å]	1.300	1.300
Number of reflections	74839
<I/σ(I)>	53.2	2.3
Completeness [%]	99.7	96.3
Redundancy	12.2	5.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	Protein: 890 micromolar in 10 mM TRIS pH 7.4. Mother Liquor: 15-30% (w/v) PEG 4K, 0.2 Li2SO4, 0.1 M TRIS pH 7.9-8.9, VAPOR DIFFUSION, HANGING DROP, temperature 295K