2Q1N
Actin Dimer Cross-linked Between Residues 41 and 374
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.2 |
Synchrotron site | ALS |
Beamline | 8.2.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-10-21 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.00 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 108.057, 71.813, 54.787 |
Unit cell angles | 90.00, 104.70, 90.00 |
Refinement procedure
Resolution | 52.260 - 2.700 |
R-factor | 0.231 |
Rwork | 0.228 |
R-free | 0.27700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2a5x |
RMSD bond length | 0.009 |
RMSD bond angle | 1.455 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 90.000 | 2.800 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.093 | 0.471 |
Number of reflections | 23524 | |
<I/σ(I)> | 9.4 | |
Completeness [%] | 99.5 | 97.1 |
Redundancy | 3.5 | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 298 | 30% MPD, 0.1M sodium acetate, 0.02 M calcium chloride, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |