2Q1M

Crystal Structure of human GITRL

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4A
Synchrotron site	NSLS
Beamline	X4A
Temperature [K]	100
Detector technology	CCD
Collection date	2006-04-08
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9795
Spacegroup name	P 63
Unit cell lengths	72.691, 72.691, 53.101
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	21.700 - 2.300
R-factor	0.217
Rwork	0.215
R-free	0.25700
Structure solution method	SAD
RMSD bond length	0.012
RMSD bond angle	1.431
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHELXD
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	21.700	2.380
High resolution limit [Å]	2.300	2.300
Rmerge	0.071
Number of reflections	7131
<I/σ(I)>	10.5
Completeness [%]	99.9	100
Redundancy	10.8	10.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.5	298	25% PEG 3350, 0.1 M Sodium Acetate pH 4.5, Vapor diffusion, Sitting drop, temperature 298K
1	VAPOR DIFFUSION, SITTING DROP	4.5	298	25% PEG 3350, 0.1 M Sodium Acetate pH 4.5, Vapor diffusion, Sitting drop, temperature 298K