2Q12
Crystal Structure of BAR domain of APPL1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-BM |
Synchrotron site | APS |
Beamline | 22-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-03-16 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.9793 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 52.985, 129.199, 36.854 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 1.790 |
R-factor | 0.214 |
Rwork | 0.212 |
R-free | 0.25500 |
Structure solution method | SAD |
RMSD bond length | 0.016 |
RMSD bond angle | 1.505 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SHARP |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.860 |
High resolution limit [Å] | 1.790 | 1.800 |
Rmerge | 0.081 | 0.440 |
Number of reflections | 23518 | |
<I/σ(I)> | 10.1 | 2.19 |
Completeness [%] | 95.7 | 78.1 |
Redundancy | 3.2 | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 0.1 M magnesium formate, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |