2Q10
RESTRICTION ENDONUCLEASE BcnI (WILD TYPE)-COGNATE DNA SUBSTRATE COMPLEX
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MPG/DESY, HAMBURG BEAMLINE BW6 |
| Synchrotron site | MPG/DESY, HAMBURG |
| Beamline | BW6 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-10-04 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 81.354, 87.551, 79.162 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.720 - 1.750 |
| R-factor | 0.176 |
| Rwork | 0.174 |
| R-free | 0.21300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2odi |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.350 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.840 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Number of reflections | 57635 | |
| <I/σ(I)> | 5.8 | 2.9 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 3.2 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 294 | 0.02 M CALCIUM CHLORIDE DIHYDRATE, 0.1 M SODIUM ACETATE TRIHYDRATE, 30% (V/V) 2-METHYL-2,4-PENTANEDIOL , pH 4.60, VAPOR DIFFUSION, SITTING DROP, temperature 294K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | CALCIUM CHLORIDE DIHYDRATE | ||
| 2 | 1 | 1 | SODIUM ACETATE TRIHYDRATE | ||
| 3 | 1 | 1 | 2-METHYL-2,4-PENTANEDIOL | ||
| 4 | 1 | 2 | CALCIUM CHLORIDE DIHYDRATE | ||
| 5 | 1 | 2 | SODIUM ACETATE TRIHYDRATE | ||
| 6 | 1 | 2 | 2-METHYL-2,4-PENTANEDIOL |
