2Q0Z

Crystal structure of Q9P172/Sec63 from Homo sapiens. Northeast Structural Genomics Target HR1979.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X12C
Synchrotron site	NSLS
Beamline	X12C
Temperature [K]	100
Detector technology	CCD
Collection date	2007-05-08
Detector	ADSC QUANTUM 210
Wavelength(s)	0.97930
Spacegroup name	P 31 2 1
Unit cell lengths	94.550, 94.550, 74.109
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 2.000
Rwork	0.196
R-free	0.22000
Structure solution method	SAD
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.970
High resolution limit [Å]	1.900	4.090	1.900
Rmerge	0.102	0.043	0.485
Number of reflections	58497
<I/σ(I)>	8.2
Completeness [%]	99.9	99.2	100
Redundancy	11.3	11.6	10.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.6	293	0.2M K/Na tartrate, 0.1M Sodium citrate pH 5.6, 1M Ammonium sulfate, 0.5M Imidazole pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K