2Q0M
Tricarbonylmanganese(I)-lysozyme complex : a structurally characterized organometallic protein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM30A |
Synchrotron site | ESRF |
Beamline | BM30A |
Detector technology | CCD |
Collection date | 2006-04-15 |
Wavelength(s) | 0.979731 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 79.542, 79.542, 36.392 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 15.000 - 1.700 |
R-factor | 0.20987 |
Rwork | 0.206 |
R-free | 0.24945 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 1.006 |
Data reduction software | XDS |
Data scaling software | XDS |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 1.700 |
Number of reflections | 11852 |
<I/σ(I)> | 20.61 |
Completeness [%] | 89.1 |
Redundancy | 3.75 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.4 | 293 | 0.9 M NaCl, 50 mM sodium acetate, pH 4.4, VAPOR DIFFUSION, HANGING DROP, temperature 293K |