2Q0I
Structure of Pseudomonas Quinolone Signal Response Protein PqsE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-05-05 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.934 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 61.100, 61.100, 145.700 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 1.570 |
| R-factor | 0.122 |
| Rwork | 0.120 |
| R-free | 0.16900 |
| RMSD bond length | 0.027 |
| RMSD bond angle | 2.089 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHELXD (& SHARP) |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.670 |
| High resolution limit [Å] | 1.570 | 1.570 |
| Rmerge | 0.051 | 0.253 |
| Number of reflections | 44378 | |
| <I/σ(I)> | 23.93 | 6.5 |
| Completeness [%] | 98.6 | 92 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.6 | 292 | 100 mM HEPES, 200 mM MgCl2, 30% PEG400, pH 7.6, VAPOR DIFFUSION, HANGING DROP, temperature 292K |
