2Q0E

Terminal uridylyl transferase 4 from Trypanosoma brucei with bound GTP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-1
Synchrotron site	SSRL
Beamline	BL9-1
Collection date	2006-07-29
Wavelength(s)	0.979
Spacegroup name	P 1 21 1
Unit cell lengths	80.788, 41.917, 106.388
Unit cell angles	90.00, 93.34, 90.00

Refinement procedure

Resolution	45.600 - 2.100
R-factor	0.23418
Rwork	0.232
R-free	0.28044
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2ikf
RMSD bond length	0.011
RMSD bond angle	1.353
Data reduction software	d*TREK
Data scaling software	d*TREK (9.2L)
Phasing software	PHASER
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	45.600	45.590	2.180
High resolution limit [Å]	2.100	4.520	2.100
Rmerge	0.076	0.023	0.464
Number of reflections	42059
<I/σ(I)>	9.8	34.3	2.4
Completeness [%]	99.7	98.9	99.8
Redundancy	3.61	3.47	3.66

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	277	100 mM sodium cacodylate, 200 mM calcium acetate, 18% PEG-8000, 4 mM MgCl2, 50 uM GTP, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K