2Q0D
Terminal uridylyl transferase 4 from Trypanosoma brucei with bound ATP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-1 |
| Synchrotron site | SSRL |
| Beamline | BL9-1 |
| Collection date | 2006-07-04 |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 80.190, 41.970, 106.420 |
| Unit cell angles | 90.00, 94.01, 90.00 |
Refinement procedure
| Resolution | 35.380 - 2.000 |
| R-factor | 0.222 |
| Rwork | 0.221 |
| R-free | 0.25400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ikf |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.547 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK (9.2L) |
| Phasing software | CCP4 (suite) |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 42.870 | 42.870 | 2.070 |
| High resolution limit [Å] | 2.000 | 4.310 | 2.000 |
| Rmerge | 0.092 | 0.028 | 0.463 |
| Total number of observations | 17513 | 15363 | |
| Number of reflections | 48171 | ||
| <I/σ(I)> | 8.5 | 29.4 | 2.3 |
| Completeness [%] | 99.5 | 99 | 97.4 |
| Redundancy | 3.56 | 3.48 | 3.27 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | 100 mM sodium cacodylate, 200 mM calcium acetate, 18% PEG-8000, 4 mM MgCl2, 50 uM ATP, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
