2Q0C

Terminal uridylyl transferase 4 from Trypanosoma brucei with bound CTP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-1
Synchrotron site	SSRL
Beamline	BL9-1
Collection date	2006-07-29
Wavelength(s)	0.979
Spacegroup name	P 1 21 1
Unit cell lengths	81.166, 42.157, 106.809
Unit cell angles	90.00, 93.66, 90.00

Refinement procedure

Resolution	45.880 - 2.200
R-factor	0.234
Rwork	0.232
R-free	0.28100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2ikf
RMSD bond length	0.013
RMSD bond angle	1.566
Data reduction software	d*TREK
Data scaling software	d*TREK (9.2L)
Phasing software	PHASER
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	45.890	45.890	2.280
High resolution limit [Å]	2.200	4.740	2.200
Rmerge	0.110	0.033	0.512
Total number of observations		13429	12544
Number of reflections	36623
<I/σ(I)>	7.4	24.6	2.1
Completeness [%]	98.2	99.1	96.8
Redundancy	3.47	3.46	3.52

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	277	100 mM sodium cacodylate, 200 mM calcium acetate, 18% PEG-8000, 4 mM MgCl2, 50 uM CTP, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K