2PZZ
2.2 A resolution crystal structure of UPF0201 protein from Methanococcus jannaschii
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X12C |
Synchrotron site | NSLS |
Beamline | X12C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-05-01 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.9793 |
Spacegroup name | P 1 |
Unit cell lengths | 46.520, 50.150, 73.820 |
Unit cell angles | 70.34, 72.61, 84.30 |
Refinement procedure
Resolution | 47.230 - 2.200 |
R-factor | 0.249 |
Rwork | 0.249 |
R-free | 0.29900 |
Structure solution method | SAD |
RMSD bond length | 0.007 |
RMSD bond angle | 1.300 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SHELXD |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.280 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.070 | 0.278 |
Number of reflections | 30128 | |
<I/σ(I)> | 14.6 | 2.4 |
Completeness [%] | 98.9 | 90.9 |
Redundancy | 7.2 | 5.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | Ammonium acetate, HEPES Buffer, PEG3350, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |