2PZX
Structure of the methuselah ectodomain with peptide inhibitor
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.2 |
Synchrotron site | ALS |
Beamline | 8.2.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-04-04 |
Detector | ADSC QUANTUM 4 |
Spacegroup name | P 42 |
Unit cell lengths | 94.233, 94.233, 173.833 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 7.990 - 3.500 |
R-factor | 0.376 |
Rwork | 0.376 |
R-free | 0.40700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1fjr |
RMSD bond length | 0.006 |
RMSD bond angle | 1.400 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 3.620 |
High resolution limit [Å] | 3.500 | 3.500 |
Rmerge | 0.155 | 0.370 |
Number of reflections | 18125 | |
<I/σ(I)> | 6.2 | |
Completeness [%] | 95.3 | 96.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.5 | 298 | 1.7 M ammonium sulfate 2% w/v PEG 400 0.1 M HEPES-KOH pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K, pH 7.50 |