2PZU
Crystal structure of Staphylococcal nuclease variant V66N/P117G/H124L/S128A at cryogenic temperature
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SEALED TUBE |
Source details | OTHER |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-01-15 |
Detector | APEX II CCD |
Wavelength(s) | 1.5418 |
Spacegroup name | P 41 |
Unit cell lengths | 48.220, 48.220, 62.970 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.100 |
R-factor | 0.208 |
Rwork | 0.211 |
R-free | 0.26500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | Staphylococcal nuclease V66E/P117G/H124L/S128A variant with b-factors set to 20.0 A^2 and residue 66 truncated to Ala; waters removed |
Data reduction software | APEX |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.200 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.014 | 0.068 |
Number of reflections | 8501 | |
<I/σ(I)> | 53.72 | 13.36 |
Completeness [%] | 100.0 | 100 |
Redundancy | 12.64 | 9.17 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.8 | 277 | 39% MPD, 0.025 M potassium phosphate, pH 7.8, VAPOR DIFFUSION, HANGING DROP, temperature 277K |