2PZS
Phi29 DNA polymerase complexed with primer-template DNA (post-translocation binary complex)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-09-25 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.10000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 216.633, 146.292, 115.054 |
| Unit cell angles | 90.00, 117.93, 90.00 |
Refinement procedure
| Resolution | 45.220 - 2.600 |
| R-factor | 0.22452 |
| Rwork | 0.219 |
| R-free | 0.27524 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | copy c of 1XHX without residues 358-394 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.239 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.690 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.152 | 0.773 |
| Number of reflections | 95762 | |
| <I/σ(I)> | 5.8 | 1 |
| Completeness [%] | 98.4 | 95.1 |
| Redundancy | 2.1 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 100 mM Tris-HCl, 20% PEG 10000, 200 mM magnesium chloride, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | sodium acetate | ||
| 2 | 1 | 1 | ammonium acetate | ||
| 3 | 1 | 1 | PEG 4000 | ||
| 4 | 1 | 2 | sodium acetate | ||
| 5 | 1 | 2 | ammonium acetate | ||
| 6 | 1 | 2 | PEG 4000 |
