2PYZ
Crystal structure of the complex of proteinase K with auramine at 1.8A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 291 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-12-10 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.54132 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 68.299, 68.299, 108.379 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 57.740 - 1.790 |
| R-factor | 0.15822 |
| Rwork | 0.156 |
| R-free | 0.20493 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ic6 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.243 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 57.740 | 1.830 |
| High resolution limit [Å] | 1.790 | 1.790 |
| Number of reflections | 22500 | |
| Completeness [%] | 95.2 | 99 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.8 | 298 | NaNO3 , pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
