2PYS
Crystal Structure of a Five Site Mutated Cyanovirin-N with a Mannose Dimer Bound at 1.8 A Resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-11-09 |
| Detector | RIGAKU RAXIS IV |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 49.226, 38.281, 55.794 |
| Unit cell angles | 90.00, 99.69, 90.00 |
Refinement procedure
| Resolution | 9.990 - 1.800 |
| R-factor | 0.171 |
| Rwork | 0.169 |
| R-free | 0.21500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1lom |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.733 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER (1.3.1 ARP/WARP 6.0) |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.220 | 1.850 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.094 | 0.244 |
| Number of reflections | 19361 | |
| <I/σ(I)> | 8.13 | 2.92 |
| Completeness [%] | 87.7 | 82.33 |
| Redundancy | 1.9 | 1.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | 30% PEG 8000, 100 MM MGSO4, 2 MM MAN2, pH 6.00, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
