2PYR

PHOTOACTIVE YELLOW PROTEIN, 1 NANOSECOND INTERMEDIATE (287K)

Experimental procedure

Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID09
Synchrotron site	ESRF
Beamline	ID09
Temperature [K]	287
Detector technology	CCD AREA DETECTOR
Collection date	1996-11
Detector	THOMPSON
Spacegroup name	P 63
Unit cell lengths	66.900, 66.900, 40.800
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 1.900
R-factor	0.18 *
R-free	0.18500 *
Data reduction software	LAUEVIEW
Data scaling software	LAUEVIEW
Phasing software	X-PLOR
Refinement software	XTALVIEW

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.570
High resolution limit [Å]	1.500	1.500
Rmerge	0.092
Number of reflections	13027
Completeness [%]	83.4	44.8
Redundancy	14	14

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	seeding *	7		pH 7.0

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	15 (mg/ml)
2	1	drop	ammonium sulfate	2.5 (M)
3	1	drop	sodium phosphate	20 (mM)
4	1	reservoir	ammonium sulfate	2.5 (M)