2PYP

PHOTOACTIVE YELLOW PROTEIN, PHOTOSTATIONARY STATE, 50% GROUND STATE, 50% BLEACHED

Experimental procedure

Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X26C
Synchrotron site	NSLS
Beamline	X26C
Temperature [K]	261
Detector technology	IMAGE PLATE
Collection date	1993-06
Detector	FUJI
Wavelength(s)	0.7, 2.0
Spacegroup name	P 63
Unit cell lengths	66.900, 66.900, 40.800
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	10.000 - 1.900
R-factor	0.204
Rwork	0.204
RMSD bond length	0.013
RMSD bond angle	1.605
Data reduction software	LaueView
Data scaling software	LaueView
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	10.000	2.100
High resolution limit [Å]	1.900	1.900
Rmerge	0.094 *
Total number of observations	56947 *
Number of reflections	10354
Completeness [%]	92.3	86
Redundancy	5.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	4.8		used to seeding, G. E. O. Borgstahl, (1995) Biochemistry, 34, 6278. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	15 (mg/ml)
2	1	drop		2.5 (M)
3	1	drop	sodium phosphate	20 (mM)
4	1	reservoir		2.5 (M)