2PYH
Azotobacter vinelandii Mannuronan C-5 epimerase AlgE4 A-module complexed with mannuronan trisaccharide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-10-23 |
| Detector | BRUKER PROTEUM R |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 169.551, 121.790, 45.068 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 57.400 - 2.700 |
| Rwork | 0.233 |
| R-free | 0.27900 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | native from other entry |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.341 |
| Data reduction software | XPREP |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 57.400 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Number of reflections | 26719 | |
| <I/σ(I)> | 10.7 | 1.9 |
| Completeness [%] | 99.2 | 93.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 1.6-1.7 M Malonate, pH 7.0-8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
