2PYC

Crystal structure of a monomeric phospholipase A2 from Russell's viper at 1.5A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X31
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X31
Temperature [K]	200
Detector technology	IMAGE PLATE
Collection date	2007-02-10
Detector	MAR scanner 345 mm plate
Wavelength(s)	0.8
Spacegroup name	P 43
Unit cell lengths	52.442, 52.442, 47.876
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.080 - 1.500
R-factor	0.18221
Rwork	0.180
R-free	0.21620
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1sv3
RMSD bond length	0.009
RMSD bond angle	1.489
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	REFMAC (5.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	52.700	1.550
High resolution limit [Å]	1.500	1.500
Number of reflections	19871
Completeness [%]	99.9	100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	298	Sodium acetate, PEG 4000, Acetonitrile, Ammonium sulphate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K