2PY9
Protein-RNA Interaction involving KH1 domain from Human Poly(C)-Binding Protein-2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-01-29 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.11588 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 92.006, 58.697, 71.496 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.000 - 2.560 |
| R-factor | 0.22038 |
| Rwork | 0.218 |
| R-free | 0.26862 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.032 |
| RMSD bond angle | 3.306 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | CNS |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 70.100 | 2.626 |
| High resolution limit [Å] | 2.560 | 2.560 |
| Rmerge | 0.102 | 0.900 |
| Number of reflections | 11660 | |
| <I/σ(I)> | 14.4 | 3.1 |
| Completeness [%] | 94.2 | 90.73 |
| Redundancy | 10.4 | 9.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.1 | 295 | 25% PEG 8000, 100mM sodium acetate, 100mM sodium cacodylate pH 6.1, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG 8000 | ||
| 2 | 1 | 1 | sodium acetate | ||
| 3 | 1 | 1 | sodium cacodylate | ||
| 4 | 1 | 2 | PEG 8000 | ||
| 5 | 1 | 2 | sodium acetate | ||
| 6 | 1 | 2 | sodium cacodylate |
