2PY5
Phi29 DNA polymerase complexed with single-stranded DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-08-15 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 54.195, 200.178, 66.989 |
| Unit cell angles | 90.00, 109.40, 90.00 |
Refinement procedure
| Resolution | 40.590 - 1.600 |
| R-factor | 0.16547 |
| Rwork | 0.164 |
| R-free | 0.19363 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | copy C of 1XHX without residues 359-394 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.201 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.660 |
| High resolution limit [Å] | 1.601 | 1.601 |
| Rmerge | 0.045 | 0.440 |
| Number of reflections | 172311 | |
| <I/σ(I)> | 49.9 | 3.7 |
| Completeness [%] | 97.8 | 92.2 |
| Redundancy | 6.9 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 0.1 M MES pH 6.5, 12% PEG 20,000, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | MES | ||
| 2 | 1 | 1 | PEG 20,000 | ||
| 3 | 1 | 2 | MES | ||
| 4 | 1 | 2 | PEG 20,000 |
