2PY2

Structure of Herring Type II Antifreeze Protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X12C
Synchrotron site	NSLS
Beamline	X12C
Temperature [K]	93
Detector technology	CCD
Collection date	2006-09-23
Wavelength(s)	1.1
Spacegroup name	P 21 21 21
Unit cell lengths	31.279, 146.415, 192.406
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 1.700
Rwork	0.209
R-free	0.24000
Structure solution method	SAD
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.700
Number of reflections	94813
Redundancy	12.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.5	293	180 mM Ammonium Sulfate, 100 mM Sodium Acetate pH 4.5, 1mM Calcium Chloride, 20% PEG 4000 and 3% D-Glucose, VAPOR DIFFUSION, HANGING DROP, temperature 293K