2PY0
Crystal structure of Cs1 pilin chimera
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-07-12 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97948 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 28.472, 52.891, 63.872 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.000 - 1.350 |
| R-factor | 0.172 |
| Rwork | 0.171 |
| R-free | 0.19700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1dzo residues 25-127 backbone atoms only. |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.561 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.385 |
| High resolution limit [Å] | 1.350 | 1.350 |
| Number of reflections | 21824 | |
| <I/σ(I)> | 42.8 | 7 |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 7.7 | 7.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.1 | 298 | 60% sat. Ammonium sulfate, 0.1 M HEPES., pH 8.1, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
