2PXY

Crystal structures of immune receptor complexes

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	CCD
Collection date	2006-02-08
Detector	ADSC QUANTUM 4
Spacegroup name	P 41 2 2
Unit cell lengths	97.603, 97.603, 175.561
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.000 - 2.230
Rwork	0.218
R-free	0.25300
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	AMoRE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.000	2.290
High resolution limit [Å]	2.200	2.200
Rmerge	0.103	0.458
Number of reflections	42263
<I/σ(I)>	23.4	3.5
Completeness [%]		100
Redundancy	4.3	4.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	295	0.2M Potassium sodium tartrate tetrahydrate, 0.1M succinic acid (pH7.0), 16% polyethylene glycol 3350, VAPOR DIFFUSION, SITTING DROP, temperature 295K