2PXS
Crystal Structure of N66D Mutant of Green Fluorescent Protein from Zoanthus sp. at 2.2 A Resolution (Mature State)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.00 |
Spacegroup name | P 62 2 2 |
Unit cell lengths | 101.510, 101.510, 271.217 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 29.300 - 2.200 |
R-factor | 0.178 |
Rwork | 0.176 |
R-free | 0.21200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | zGFP506 |
RMSD bond length | 0.018 |
RMSD bond angle | 1.793 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 30.000 | 30.000 | 2.280 |
High resolution limit [Å] | 2.200 | 4.730 | 2.200 |
Rmerge | 0.066 | 0.048 | 0.663 |
Number of reflections | 42936 | ||
<I/σ(I)> | 11.3 | ||
Completeness [%] | 99.8 | 98.8 | 100 |
Redundancy | 14.4 | 13.3 | 14.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 0.1 M bis-tris propane, 1.8 M tri-ammonium citrate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |