2PXF
Variant 5 of Ribonucleoprotein Core of the E. Coli Signal Recognition Particle
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 113 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-01-01 |
| Detector | RIGAKU RAXIS |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 132.320, 77.450, 32.690 |
| Unit cell angles | 90.00, 94.15, 90.00 |
Refinement procedure
| Resolution | 40.000 - 2.000 |
| Rwork | 0.268 |
| R-free | 0.31200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK (9.4L) |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 38.720 | 38.720 | 2.070 |
| High resolution limit [Å] | 2.000 | 4.310 | 2.000 |
| Rmerge | 0.150 | 0.074 | 0.526 |
| Total number of observations | 16866 | 14717 | |
| Number of reflections | 21241 | ||
| <I/σ(I)> | 6.1 | 21.6 | 2.7 |
| Completeness [%] | 95.2 | 97.6 | 90.1 |
| Redundancy | 7.56 | 7.11 | 7.26 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 293 | 20Mm NaOH-MES pH 5.6, 200mM KCl, 8% Isopropanol, 4mM Cobalt Hexamine, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 293 | 20Mm NaOH-MES pH 5.6, 200mM KCl, 8% Isopropanol, 4mM Cobalt Hexamine, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | NaOH-MES | ||
| 2 | 1 | 1 | KCl | ||
| 3 | 1 | 1 | Isopropanol | ||
| 4 | 1 | 1 | Cobalt Hexamine | ||
| 5 | 1 | 1 | H2O | ||
| 6 | 1 | 2 | NaOH-MES | ||
| 7 | 1 | 2 | KCl | ||
| 8 | 1 | 2 | Isopropanol | ||
| 9 | 1 | 2 | Cobalt Hexamine |
