2PX3
Crystal structure of FlhF complexed with GTP/Mg(2+)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-11-29 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.979 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 80.965, 80.965, 126.137 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.760 - 3.200 |
Rwork | 0.250 |
R-free | 0.31000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | G domain of FtsY from Sulfolobus solfataricus |
RMSD bond length | 0.008 |
RMSD bond angle | 1.900 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 68.200 | 3.310 |
High resolution limit [Å] | 3.200 | 3.200 |
Number of reflections | 7364 | |
<I/σ(I)> | 19.4 | 4.2 |
Completeness [%] | 99.9 | 99.9 |
Redundancy | 7.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 10-13 % (w/v) PEG6000, 4% (v/v) 2-methyl-2,4-pentanediol, 0.1 M MOPS, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |