2PX1
crystal structure of the complex of bovine lactoferrin C-lobe with Ribose at 2.5 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X31 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X31 |
| Temperature [K] | 273 |
| Detector technology | CCD |
| Collection date | 2007-03-28 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.8088 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 59.906, 49.631, 64.705 |
| Unit cell angles | 90.00, 105.88, 90.00 |
Refinement procedure
| Resolution | 19.960 - 2.500 |
| R-factor | 0.204 |
| Rwork | 0.204 |
| R-free | 0.22800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1nkx |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.900 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.540 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Number of reflections | 12819 | |
| <I/σ(I)> | 2.1 | 13.4 |
| Completeness [%] | 99.5 | 92 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 0.01M znso4, 25% PEG, Monomethyl ether 550, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
