2PX0

Crystal structure of FlhF complexed with GMPPNP/Mg(2+)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-2
Synchrotron site	ESRF
Beamline	ID14-2
Temperature [K]	100
Detector technology	CCD
Collection date	2005-08-31
Detector	ADSC QUANTUM 4
Wavelength(s)	0.933
Spacegroup name	P 1 21 1
Unit cell lengths	104.649, 111.376, 112.089
Unit cell angles	90.00, 89.78, 90.00

Refinement procedure

Resolution	62.970 - 3.000
R-factor	0.254
Rwork	0.254
R-free	0.32800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	NG domain of FlhF/GTP ( residues: 109 - 215 and 218 - 366)
RMSD bond length	0.007
RMSD bond angle	1.800
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	111.800	3.160
High resolution limit [Å]	3.000	3.000
Number of reflections	51671
<I/σ(I)>	12.3	2.2
Completeness [%]	99.0	99.8
Redundancy	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	13 % (w/v) PEG6000, 4 % (v/v) 2-methyl-2,4-pentanediol, 0.1M MOPS, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K