2PX0
Crystal structure of FlhF complexed with GMPPNP/Mg(2+)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-2 |
Synchrotron site | ESRF |
Beamline | ID14-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-08-31 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.933 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 104.649, 111.376, 112.089 |
Unit cell angles | 90.00, 89.78, 90.00 |
Refinement procedure
Resolution | 62.970 - 3.000 |
R-factor | 0.254 |
Rwork | 0.254 |
R-free | 0.32800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | NG domain of FlhF/GTP ( residues: 109 - 215 and 218 - 366) |
RMSD bond length | 0.007 |
RMSD bond angle | 1.800 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 111.800 | 3.160 |
High resolution limit [Å] | 3.000 | 3.000 |
Number of reflections | 51671 | |
<I/σ(I)> | 12.3 | 2.2 |
Completeness [%] | 99.0 | 99.8 |
Redundancy | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 13 % (w/v) PEG6000, 4 % (v/v) 2-methyl-2,4-pentanediol, 0.1M MOPS, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |