2PWY
Crystal Structure of a m1A58 tRNA methyltransferase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-09-15 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.934 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 90.440, 96.795, 140.584 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.670 - 1.700 |
| R-factor | 0.181 |
| Rwork | 0.180 |
| R-free | 0.20900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1i9g |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.265 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.168 | 1.790 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.106 | 0.674 |
| Total number of observations | 71441 | |
| Number of reflections | 67981 | |
| <I/σ(I)> | 5.9 | 2.9 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7.3 | 7.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 292 | 2.0 M Ammonium Sulfate, 5% isopropanol, 2 mM S-adenosylhomocysteine, 1.eq tRNAiMet, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 292K |
