2PWX
Crystal structure of G11A mutant of SARS-CoV 3C-like protease
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-01-15 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 34.147, 66.052, 129.030 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 2.500 |
| Rwork | 0.244 |
| R-free | 0.29600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | MOLREP |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.660 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.144 | 0.306 |
| Total number of observations | 4895 | |
| Number of reflections | 10537 | |
| <I/σ(I)> | 4.2 | 1.9 |
| Completeness [%] | 98.9 | 100 |
| Redundancy | 4.59 | 4.71 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 277 | 0.1M MES pH 6.2, 10% PEG6000, 1mM DTT, 5% DMSO, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
