2PWQ
Crystal structure of a putative ubiquitin conjugating enzyme from Plasmodium yoelii
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E+ SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-01-05 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 48.556, 48.556, 179.502 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 24.270 - 1.900 |
| R-factor | 0.26941 |
| Rwork | 0.267 |
| R-free | 0.30767 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2f4z |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.606 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.930 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 19987 | |
| <I/σ(I)> | 59.8 | 2.5 |
| Completeness [%] | 98.5 | 87.7 |
| Redundancy | 9.7 | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 20% PEG 3350, 0.2 M Sodium dihydrogen phosphate, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
