2PWO
Crystal Structure of HIV-1 CA146 A92E Psuedo Cell
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-04-08 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.1000 |
| Spacegroup name | P 1 |
| Unit cell lengths | 46.225, 48.208, 58.928 |
| Unit cell angles | 83.01, 71.28, 87.43 |
Refinement procedure
| Resolution | 30.030 - 1.450 |
| R-factor | 0.173 |
| Rwork | 0.170 |
| R-free | 0.21600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | CA146 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.664 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.500 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Rmerge | 0.056 | 0.294 |
| Number of reflections | 77217 | |
| <I/σ(I)> | 14 | 2 |
| Completeness [%] | 91.0 | 61.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 294 | 24% PEG 4500, 0.60 M MgCl2, 100 mM Tris-HCl, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 294K |
