2PWN

Crystal structure of BET3 homolog (13277653) from Mus musculus at 2.04 A resolution

Replaces:  1VPG

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	100
Detector technology	CCD
Collection date	2004-09-26
Detector	ADSC QUANTUM 210
Wavelength(s)	0.99987, 0.97969, 0.97957
Spacegroup name	P 31 2 1
Unit cell lengths	77.533, 77.533, 56.591
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	23.156 - 2.040
R-factor	0.185
Rwork	0.183
R-free	0.23700
Structure solution method	MAD
RMSD bond length	0.016
RMSD bond angle	1.381
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	SHELX
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	23.156	23.170	2.090
High resolution limit [Å]	2.040	9.120	2.040
Rmerge	0.070	0.051	0.687
Total number of observations		725	4286
Number of reflections	12800
<I/σ(I)>	14	9.8	2.1
Completeness [%]	99.8	90.9	100
Redundancy	5.5	4.8	4.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	NANODROP, 0.4M NH4H2PO3, VAPOR DIFFUSION, SITTING DROP, temperature 277K