2PWH
Crystal structure of the trehalulose synthase MutB from Pseudomonas mesoacidophila MX-45
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM30A |
Synchrotron site | ESRF |
Beamline | BM30A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-04-18 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.9803 |
Spacegroup name | P 1 |
Unit cell lengths | 63.580, 71.540, 81.750 |
Unit cell angles | 67.66, 73.04, 70.44 |
Refinement procedure
Resolution | 19.560 - 2.000 |
R-factor | 0.172 |
Rwork | 0.172 |
R-free | 0.21500 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 1zja |
RMSD bond length | 0.005 |
RMSD bond angle | 1.200 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | CNS |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.600 | 2.100 |
High resolution limit [Å] | 2.000 | 2.000 |
Number of reflections | 80624 | |
<I/σ(I)> | 8.9 | 2.84 |
Completeness [%] | 96.6 | |
Redundancy | 2 | 2.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 290 | 12% PEG20000, 0.1M Na cacodylate, 0.01M L-cysteine, pH 6, VAPOR DIFFUSION, HANGING DROP, temperature 290K |